A novel fluorescent GSH-adduct binds to the NMDA receptor.
J Neurosci Methods
; 93(1): 21-6, 1999 Oct 30.
Article
em En
| MEDLINE
| ID: mdl-10598861
ABSTRACT
In an attempt to develop various fluorescent probes to label glutathione (GSH) receptors, we have serendipitously synthesized a probe that binds to and antagonizes the NMDA receptor. Probe 1, a GSH adduct, displaces the competitive NMDA antagonist [3H]-CGP 39653 with a higher affinity than NMDA or cysteine in rat synaptic membranes. In recording experiments from a rat cortical 'wedge' preparation, Probe 1 reversibly blocks both NMDA- and cysteine-induced depolarization. In mixed astrocyte-neuron tissue culture preparations, Probe 1 labels parts of both cell bodies as well as processes. The present data suggest that Probe 1 binds to the NMDA receptor and antagonizes channel function.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Receptores de N-Metil-D-Aspartato
/
Receptores de Peptídeos
/
Corantes Fluorescentes
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Neurosci Methods
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Canadá