The vaccinia virus 39-kDa protein forms a stable complex with the p4a/4a major core protein early in morphogenesis.
Virology
; 265(2): 375-86, 1999 Dec 20.
Article
em En
| MEDLINE
| ID: mdl-10600608
ABSTRACT
The vaccinia virus (VV) 39-kDa protein, the product of the A4L gene, is a highly antigenic protein of the viral core. Pulse-chase and immunoprecipitation experiments have shown that the 39-kDa protein interacts with p4a (encoded by the A10L gene), the precursor of the most abundant virion protein. This interaction is maintained with the processed 4a form that arises during virion maturation. The controlled disruption of mature viral particles showed that the 39-kDa and 4a proteins are tightly bound within the virion. Immunoelectron microscopy showed that both proteins first localize within the cytoplasm and later accumulate inside the viral factories, reaching these locations via a mechanism apparently unrelated to cellular membranes. Double labeling experiments showed a colocalization of both proteins in all virus-induced structures.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vaccinia virus
/
Proteínas Virais
/
Proteínas do Core Viral
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Virology
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Espanha