[Western blotting of RStV gene products in rice and insects].
Yi Chuan Xue Bao
; 26(5): 512-7, 1999.
Article
em Zh
| MEDLINE
| ID: mdl-10665229
ABSTRACT
The NS3 and NC protein genes encoded by RNA3 of RStV, the NCP and NSvc4 protein genes encoded by RNA4 were subcloned into the E. coli expression vector pGEX3X to express four groups of fusion protein under IPTG induction. These fusion proteins were used to immunize rabbits to raise antisera. The antisera against the E. coli-expressed proteins were available for probing the presence of the viral gene products in both rice plant and insect hosts. The expected gene products can be probed only in diseased rice plant with NCP antiserum and the corresponding products detected in both plant and RStV particle preparation with NC antiserum. The viral gene products probed by NS3 and NSvc4 antisera were different from the expected ones in size.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vírus de Plantas
/
Oryza
/
Proteínas Virais
/
Proteínas Recombinantes de Fusão
Limite:
Animals
Idioma:
Zh
Revista:
Yi Chuan Xue Bao
Ano de publicação:
1999
Tipo de documento:
Article