Crystallization and preliminary X-ray diffraction studies on the N-utilizing substance-B (NusB) from Mycobacterium tuberculosis.
Acta Crystallogr D Biol Crystallogr
; 56(Pt 1): 64-6, 2000 Jan.
Article
em En
| MEDLINE
| ID: mdl-10666629
ABSTRACT
N-utilizing substance B (NusB) is a protein which forms part of a complex assembly in transcriptional antitermination in Mycobacterium tuberculosis. It forms a heterodimer with the product of the NusE gene (identical to the ribosomal protein S10) and mediates the process of transcriptional antitermination by forming the core complex with the nut site of the ribosomal RNA along with other protein factors. NusB has been cloned and overexpressed in Escherichia coli and crystallized using the hanging-drop vapour-diffusion method. The space group is P2(1)2(1)2(1), with unit-cell parameters a = 46.6, b = 64.2, c = 90.1 A. A native data set complete to 1.6 A resolution has been collected from a single crystal.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Fatores de Transcrição
/
Proteínas de Escherichia coli
/
Mycobacterium tuberculosis
Idioma:
En
Revista:
Acta Crystallogr D Biol Crystallogr
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Reino Unido