A polymorphism in the mouse crg-2/IP-10 gene complicates chemokine gene expression analysis using a commercial ribonuclease protection assay.
J Immunol Methods
; 234(1-2): 149-51, 2000 Feb 03.
Article
em En
| MEDLINE
| ID: mdl-10669779
ABSTRACT
The use of multiprobe RPAs is becoming an increasingly popular method for the detection and quantitation of RNA levels in cells and tissues. Here we report that due to a polymorphism in the 3'-noncoding region of the mouse Crg-2/IP-10 gene, the mCK-5 chemokine probe set available from Pharmingen can yield aberrant signal patterns with RNA samples from BALB/c, MRL and possibly other mouse strains that may lead to false conclusions regarding expression of the Crg-2/IP-10 and MCP-1 genes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polimorfismo Genético
/
Monocinas
/
Quimiocinas CXC
Limite:
Animals
Idioma:
En
Revista:
J Immunol Methods
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Alemanha