Modulation of ribonuclease P activity by calcipotriol.
Eur J Biochem
; 267(4): 1173-7, 2000 Feb.
Article
em En
| MEDLINE
| ID: mdl-10672028
ABSTRACT
The effects of cholesterol, 7-dehydrocholesterol, vitamin D3 and several synthetic vitamin D3 analogs on ribonuclease P (RNase P) were investigated using a cell-free system from the slime mold Dictyostelium discoideum. RNase P is an ubiquitous and essential enzyme that endonucleolytically cleaves all tRNA precursors to produce the mature 5' end. Among the compounds tested, only calcipotriol was capable of affecting RNase P activity, and revealed a bimodal action at the kinetic phase of the reaction. Depending on the concentration of the drug, both activation and inhibition of tRNA maturation were observed, indicating that calcipotriol may have a direct effect on tRNA biogenesis, possibly associated with the presence of a highly reactive small ring on the side chain of its molecule.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Calcitriol
/
RNA Catalítico
/
Dictyostelium
/
Endorribonucleases
Limite:
Animals
Idioma:
En
Revista:
Eur J Biochem
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Grécia