Peritoneal mesothelial cells produce complement factors and express CD59 that inhibits C5b-9-mediated cell lysis.
Adv Perit Dial
; 15: 253-7, 1999.
Article
em En
| MEDLINE
| ID: mdl-10682113
ABSTRACT
The CD59 membrane protein confers protection from C5b-9-mediated cell lysis. Because evidence exists for complement (C) activation and generation of C5b-9 in the peritoneal cavity during chronic peritoneal dialysis (CPD), we investigated, on mesothelial cell (MC) lines, the expression of CD59 and the production of C components. Four MC lines were obtained from children on CPD, and two from non uremic children. CD59 expression on MCs was investigated with anti-CD59 monoclonal antibody (mAb) and polyclonal goat immunoglobulin G (IgG). MC lines were positive for staining with anti-CD59 mAb. Western blotting analysis of MC membrane demonstrated a band with the same molecular weight as CD59. Incubation of MC with anti-CD59 mAb abrogated the protective effect of CD59 (100% cytotoxicity). C3, C4, and C6 were detected in the supernatants of MC; in non uremic MC supernatants, C5, C7, C8, and C9 were also detectable, and C4 concentration was tenfold higher. CD59 expression confers to MCs protection from C5b-9-mediated lysis. MCs produce C factors. These findings suggest that production of complement components and expression of CD59 on MCs could play a role both in peritoneal cavity infection (decreased complement production) and in peritoneal membrane damage (decreased CD59 expression and reduced remesothelialization owing to MC lysis).
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peritônio
/
Proteínas do Sistema Complemento
/
Complexo de Ataque à Membrana do Sistema Complemento
/
Diálise Peritoneal
/
Antígenos CD59
Limite:
Child
/
Humans
Idioma:
En
Revista:
Adv Perit Dial
Assunto da revista:
NEFROLOGIA
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Itália
País de publicação:
CA
/
CANADA
/
CANADÁ