Disruption of microtubules in living cells by tyrphostin AG-1714.
Cell Motil Cytoskeleton
; 45(3): 223-34, 2000 Mar.
Article
em En
| MEDLINE
| ID: mdl-10706777
ABSTRACT
Tyrphostin AG-1714 and several related molecules with the general structure of nitro-benzene malononitrile (BMN) disrupt microtubules in a large variety of cultured cells. This process can be inhibited by the stabilization of microtubules with taxol or by pretreatment of the cells with pervanadate, which inhibits tyrosine phosphatases and increases the overall levels of phosphotyrosine in cells. Unlike other microtubule-disrupting drugs such as nocodazole or colchicine, tyrphostin AG-1714 does not interfere with microtubule polymerization or stability in vitro, suggesting that the effect of this tyrphostin on microtubules is indirect. These results imply an involvement of protein tyrosine phosphorylation in the regulation of overall microtubule dynamics. Tyrphostins of AG-1714 type could thus be powerful tools for the identification of such microtubule regulatory pathways.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tirfostinas
/
Microtúbulos
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Cell Motil Cytoskeleton
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Israel