Sequencing and expression of additional xylanase genes from the hyperthermophile Thermotoga maritima FjSS3B.1.
Appl Environ Microbiol
; 66(4): 1532-7, 2000 Apr.
Article
em En
| MEDLINE
| ID: mdl-10742238
ABSTRACT
Two genes, xynB and xynC, coding for xylanases were isolated from Thermotoga maritima FjSS3B.1 by a genomic-walking-PCR technique. Sequencing of the genes showed that they encode multidomain family 10 xylanases. Only XynB exhibited activity against xylan substrates. The temperature optimum (87 degrees C) and pH optimum (pH 6.5) of XynB are different from the previously reported xylanase, XynA (also a family 10 enzyme), from this organism. The catalytic domain expressed without other domains has a lower temperature optimum, is less thermostable, and has optimal activity at pH 6.5. Despite having a high level of sequence similarity to xynB, xynC appears to be nonfunctional since its encoded protein did not show significant activity on xylan substrates.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bactérias
/
Xilosidases
/
Genes Bacterianos
Idioma:
En
Revista:
Appl Environ Microbiol
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Nova Zelândia