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Endothelial cell seeding of (heparinized) collagen matrices: effects of bFGF pre-loading on proliferation (after low density seeding) and pro-coagulant factors.
Wissink, M J; Beernink, R; Scharenborg, N M; Poot, A A; Engbers, G H; Beugeling, T; van Aken, W G; Feijen, J.
Afiliação
  • Wissink MJ; Institute for Biomedical Technology, Polymer Chemistry and Biomaterials Group, Department of Chemical Technology, University of Twente, P.O. Box 217, 7500 AE, Enschede, The Netherlands.
J Control Release ; 67(2-3): 141-55, 2000 Jul 03.
Article em En | MEDLINE | ID: mdl-10825549
Endothelial cell seeding to improve the performance of small-diameter vascular grafts requires a suitable substrate, such as crosslinked collagen. In addition to providing a suitable substrate for adhesion and growth of endothelial cells, proliferation of seeded endothelial cells can be enhanced by local, sustained release of basic fibroblast growth factor (bFGF, a heparin-binding growth factor for endothelial cells). We have previously shown that collagen crosslinked using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide (EDC) and N-hydroxysuccinimide (NHS) supports adhesion and proliferation of human umbilical vein endothelial cells (HUVECs). In the present study, HUVECs were seeded on (heparinized) EDC/NHS-crosslinked collagen, pre-loaded with bFGF. Proliferation of HUVECs on (heparinized) crosslinked collagen increased with increasing amounts of pre-loaded bFGF. The minimal cell-seeding density required for proliferation proved to be very low after pre-loading the substrates with bFGF, and was 4-fold lower for heparinized crosslinked collagen compared to crosslinked collagen (250 versus 1000 cells/cm(2)). Pro-coagulant properties (von Willebrand factor secretion and tissue factor expression) of HUVECs seeded on (heparinized) crosslinked collagen, with or without pre-loading of bFGF, were comparable to those of HUVECs on TCPS. It is concluded that heparinized, EDC/NHS-crosslinked collagen pre-loaded with bFGF is a candidate matrix for in vivo endothelial cell seeding of synthetic vascular graft materials.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Endotélio Vascular / Coagulantes / Heparina / Fator 2 de Crescimento de Fibroblastos / Colágeno Limite: Humans Idioma: En Revista: J Control Release Assunto da revista: FARMACOLOGIA Ano de publicação: 2000 Tipo de documento: Article País de afiliação: Holanda País de publicação: Holanda
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Endotélio Vascular / Coagulantes / Heparina / Fator 2 de Crescimento de Fibroblastos / Colágeno Limite: Humans Idioma: En Revista: J Control Release Assunto da revista: FARMACOLOGIA Ano de publicação: 2000 Tipo de documento: Article País de afiliação: Holanda País de publicação: Holanda