Analysis of carbohydrates using liquid chromatography--surface plasmon resonance immunosensing systems.
Anal Biochem
; 281(2): 151-8, 2000 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-10870830
ABSTRACT
An immunosensing system based on surface plasmon resonance (SPR) was used for on-line detection and characterization of carbohydrate molecules separated by high-performance liquid chromatography. These analytes, with or without serum, were continuously separated and analyzed in the combined liquid chromatography-surface plasmon resonance (LC-SPR) system. By using weak and readily reversible monoclonal antibodies, the SPR system allowed specific on-line monitoring of the substances. To increase the specificity of the immunosensor, nonrelevant antibodies were used as reference in a serial flow cell. The sensitivity of the LC-SPR system was dependent on molecular weight of the carbohydrate, affinity of binding, and design of the sensor.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Carboidratos
/
Cromatografia Líquida de Alta Pressão
/
Ressonância de Plasmônio de Superfície
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Suécia