Method for cloning in vivo targets of the Egr-1 transcription factor.
Biotechniques
; 29(1): 162-9, 2000 Jul.
Article
em En
| MEDLINE
| ID: mdl-10907091
ABSTRACT
A methodology is described that allows the in vivo trapping of transcription factors to their target regulatory elements in multiple genes simultaneously. Cross-linking using formaldehyde is the first of several steps to isolate, purify, clone and characterize multiple gene promoter DNA fragments. The example that we use indicates that the TGF beta 1 gene is a direct target induced by Egr-1 in HT1080 cells that express constitutive Egr-1, thus explaining the growth retardation that follows Egr-1 expression. The genes identified using this procedure reflect the specific activities of Egr-1 at that moment in the cell and provide a method for confirmation of genes that are the direct targets of Egr-1 action.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
/
Clonagem Molecular
/
Proteínas Imediatamente Precoces
/
Proteínas de Ligação a DNA
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Biotechniques
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Estados Unidos