A role for GATA transcription factors in the androgen regulation of the prostate-specific antigen gene enhancer.
Mol Cell Endocrinol
; 167(1-2): 43-53, 2000 Sep 25.
Article
em En
| MEDLINE
| ID: mdl-11000519
ABSTRACT
The transgenic mouse line Ggamma/T-15 containing the fetal globin promoter linked to SV40 T antigen unexpectedly results in androgen-independent prostate carcinomas. Given the key role of GATA-1 transcription factor in fetal globin gene promoter activity, we investigated whether specific GATA family members are expressed in the prostate and whether they can regulate prostate-specific genes. We found that GATA-2 and -3 are the predominant GATA family members expressed in human and mouse prostate and that GATA mRNA levels are not regulated by androgen. We identified six GATA sites flanking an androgen-response element located in the far-upstream enhancer of the prostate-specific antigen (PSA) gene. These GATA sites are targets for GATA factors and are essential for optimal androgen induction of transfected PSA enhancer/promoter plasmids in LNCaP, a PSA and androgen receptor expressing human prostate cancer cell line. Our results suggest that prostatic GATA-2 and -3 are involved in the androgen regulation of the PSA gene.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
/
Transativadores
/
Elementos Facilitadores Genéticos
/
Regiões Promotoras Genéticas
/
Antígeno Prostático Específico
/
Proteínas de Ligação a DNA
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
Mol Cell Endocrinol
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Estados Unidos