High-affinity monoclonal antibodies to PED/PEA-15 generated using 5 microg of DNA.
Hybridoma
; 19(4): 297-302, 2000 Aug.
Article
em En
| MEDLINE
| ID: mdl-11001402
ABSTRACT
Class-switched, affinity-matured murine monoclonal antibody (MAb) producing cell lines reactive with PED/PEA-15 were generated and isolated in less than 4 weeks following polynucleotide immunizations using only 5 microg of DNA in conjunction with the Powderject gene gun. Somatic fusions of peripheral lymph node cells were performed 13 days after initiating delivery of DNA encoding the target antigen. The data presented demonstrates the rapid production, identification, and characterization of class-switched, affinity-matured MAbs that bind PED/PEA-15. The reported strategy enabled the rapid development of MAbs that are useful in enzyme-linked immunoadsorbent assay (ELISA), Western blotting, and immunoprecipitations.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfoproteínas
/
DNA
/
Antígenos de Histocompatibilidade Classe I
/
Anticorpos Antinucleares
/
Anticorpos Monoclonais
/
Afinidade de Anticorpos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Hybridoma
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Estados Unidos