A rare mutation in the amelogenin gene and its potential investigative ramifications.
J Forensic Sci
; 45(5): 1016-9, 2000 Sep.
Article
em En
| MEDLINE
| ID: mdl-11005174
ABSTRACT
Over the past few years, the Australian forensic science community has adopted a common methodology and technology in the application of DNA profiling for investigative and forensic purposes. The ultimate objective of this initiative is the establishment of a national DNA database similar to that used in the UK. An integral part of this methodology is the use of "Profiler Plus," a nonaplex of STRs combined with amelogenin, a locus utilized for sex determination. This paper reports the results from a case where a mutation in the annealing region of the amelogenin primers appears to have resulted in the failure to amplify the amelogenin Y-homolog from a phenotypically normal male. The result was confirmed using two different primer sets that amplify different regions of the amelogenin gene. This situation suggests that the genetic determination of sex based on the amelogenin sequences from specimens of unknown origin, such as crime scene samples, should not be considered infallible.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromossomo Y
/
Impressões Digitais de DNA
/
Processos de Determinação Sexual
/
Proteínas do Esmalte Dentário
Limite:
Humans
/
Male
Idioma:
En
Revista:
J Forensic Sci
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Austrália