Site-specific and directional gene replacement mediated by Cre recombinase.
J Immunol Methods
; 244(1-2): 185-93, 2000 Oct 20.
Article
em En
| MEDLINE
| ID: mdl-11033031
ABSTRACT
A novel method for the site-specific introduction of genes into eukaryotic cells using the prokaryotic Cre-LoxP recombination system is presented. Cre recombinase catalyzes recombination between two LoxP sites or between two mutant LoxP 511 sites. However, recombination is not catalyzed between a LoxP and a LoxP 511 site. We now demonstrate that it is possible to catalyze accurate exchange between two DNA segments each flanked by a LoxP and a LoxP 511 site. In the example presented, expression of the Cre recombinase resulted in the replacement of a murine IgA constant region gene with a LoxP site at the 5' end and a LoxP 511 site at the 3' end by a human IgA constant region gene flanked by the same wild type and mutant LoxP sites. This method provides a novel approach for the site-specific substitution of specific genes.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Recombinação Genética
/
Proteínas Virais
/
Integrases
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Immunol Methods
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Estados Unidos