Gram-scale purification of methionyl-tRNA and tyrosyl-tRNA synthetases from Escherichia coli.
Eur J Biochem
; 59(2): 327-33, 1975 Nov 15.
Article
em En
| MEDLINE
| ID: mdl-1107028
ABSTRACT
A procedure is presented for the purification of 3 g of homogeneous methionyl-tRNA synthetase and 1 g of homogeneous tyrosyl-tRNA synthetase from 50 kg batches of Escherichia coli. The procedure permits the isolation of many enzymes simultaneously and the elution positions of seven other aminoacyl-tRNA synthetases, catalase, rhodanese, phosphofructokinase, elongation factor Tu and cytochrome b-562 are indicated. The problems of extraction work on this scale are discussed.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tirosina-tRNA Ligase
/
Escherichia coli
/
Aminoacil-tRNA Sintetases
/
Metionina tRNA Ligase
Idioma:
En
Revista:
Eur J Biochem
Ano de publicação:
1975
Tipo de documento:
Article