Phase boundaries in mixtures of membrane-forming amphiphiles and micelle-forming amphiphiles.
Biochim Biophys Acta
; 1508(1-2): 1-19, 2000 Nov 23.
Article
em En
| MEDLINE
| ID: mdl-11090815
ABSTRACT
The phase behavior of mixtures of phospholipids and detergents in aqueous solutions is an issue of basic importance for understanding the solubilization and reconstitution of biological membranes. We review the existing knowledge on the compositionally induced reversible transformation of phospholipid bilayers into lipid-detergent mixed micelles. First, we describe the experimental protocols used for preparation of such mixtures and emphasize the scope and limitations of the various techniques used for evaluation of the microstructures of the self-assembled amphiphiles in the mixture. Subsequently, we interpret the existing data in terms of the spontaneous curvature of the amphiphiles and the finite size of the mixed micelles. These considerations lead to a general description of the phase behavior, which forms the basis for a rational approach to solubilization and reconstitution experiments.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tensoativos
/
Membranas
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Israel