Characterization of a cDNA encoding a 72 kDa heat shock cognate protein (Hsc72) from the Pacific oyster, Crassostrea gigas.
DNA Seq
; 11(3-4): 265-70, 2000.
Article
em En
| MEDLINE
| ID: mdl-11092738
ABSTRACT
A full-length cDNA encoding a 72 kDa heat shock cognate protein (Hsc72) was isolated from a Crassostrea gigas hemocyte library. This cDNA is 75% identical to a human hsc70 cDNA. C. gigas cDNA contains a 659 amino acid open reading frame encoding a 72 kDa protein which is 87% identical to a human Hsc70 protein. Northern blotting indicated that even if the hsc72 gene was constitutively expressed in oyster hemocytes, it could be stimulated by heat shock in vitro as well as in vivo. Homologies observed both at the cDNA and protein levels, as well as the expression pattern of the hsc72 mRNA, support our conclusion that the isolated cDNA clone corresponds to an oyster heat shock cognate gene.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ostreidae
/
Proteínas de Choque Térmico
Limite:
Animals
/
Humans
Idioma:
En
Revista:
DNA Seq
Assunto da revista:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
França