Keratin mRNA for detecting micrometastasis in cervical lymph nodes of oral cancer.
Cancer Lett
; 160(1): 115-23, 2000 Nov 10.
Article
em En
| MEDLINE
| ID: mdl-11098092
ABSTRACT
We studied three keratin (K) gene candidates, K13, K19, and K20 mRNAs, for detecting micrometastases in cervical lymph nodes (LNs) by reverse transcriptase-polymerase chain reaction (RT-PCR). Of 166 histologically metastasis-negative nodes, 24 micrometastatic LNs (14. 4%) were detected based on K13 gene expression. Keratin 19 mRNA is an inadequate marker for the genetic diagnosis due to not only illegitimate gene expression from lymphatic tissue but also gene expression from the ectopic salivary gland. Keratin 20 mRNA showed low sensitivity. It is suggested that K13 mRNA may be a promising tumor marker among these keratin genes for detecting the micrometastases in cervical LNs of oral cancer.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
/
Neoplasias Bucais
/
Queratinas
/
Metástase Linfática
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Cancer Lett
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Japão