The isolation and properties of the dimeric subunit of concanavalin A.
J Protein Chem
; 19(5): 353-9, 2000 Jul.
Article
em En
| MEDLINE
| ID: mdl-11131142
ABSTRACT
Concanavalin A (Con A) was dissociated into dimeric and monomeric subunits by incubation at 37 degrees C in acetate buffer of pH 3.8 containing 0.5% sodium dodecyl sulfate. The dimer was isolated in pure form by a density gradient ultracentrifugation method. Several properties of the dimer were determined including the formation of a precipitin with anti-Con A antibodies, the molecular weight, the lack of a binding site for glycogen, the lack of mitogenic activity for spleen lymphocytes, and the lack of inhibition by alpha-methyl D-glucoside. The latter findings differ from results reported by other investigators.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Concanavalina A
Idioma:
En
Revista:
J Protein Chem
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Estados Unidos