SpKrl: a direct target of beta-catenin regulation required for endoderm differentiation in sea urchin embryos.
Development
; 128(3): 365-75, 2001 Feb.
Article
em En
| MEDLINE
| ID: mdl-11152635
ABSTRACT
Localization of nuclear beta-catenin initiates specification of vegetal fates in sea urchin embryos. We have identified SpKrl, a gene that is activated upon nuclear entry of beta-catenin. SpKrl is upregulated when nuclear beta-catenin activity is increased with LiCl and downregulated in embryos injected with molecules that inhibit beta-catenin nuclear function. LiCl-mediated SpKrl activation is independent of protein synthesis, indicating that SpKrl is a direct target of beat-catenin and TCF. Embryos in which SpKrl translation is inhibited with morpholino antisense oligonucleotides lack endoderm. Conversely, SpKrl mRNA injection rescues some vegetal structures in beta-catenin-deficient embryos. SpKrl negatively regulates expression of the animalizing transcription factor, SpSoxB1. We propose that SpKrl functions in patterning the vegetal domain by suppressing animal regulatory activities.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ouriços-do-Mar
/
Fatores de Transcrição
/
Diferenciação Celular
/
Transativadores
/
Proteínas do Citoesqueleto
/
Proteínas de Peixe-Zebra
/
Endoderma
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Development
Assunto da revista:
BIOLOGIA
/
EMBRIOLOGIA
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Estados Unidos