Production of antimicrobial defensin in Nicotiana benthamiana with a potato virus X vector.
Mol Plant Microbe Interact
; 14(2): 111-5, 2001 Feb.
Article
em En
| MEDLINE
| ID: mdl-11204773
ABSTRACT
A recombinant plasmid, pTXS.TH, was constructed to express the gene-encoding wasabi (Wasabia japonica) defensin with the potato virus X (PVX) vector. pTXS.TH allows the expression of defensin in the host Nicotiana benthamiana, and the defensin protein WT1 can be purified from virus-infected leaves by heat treatment and affinity chromatography. WT1 exhibits strong antifungal activity toward the phytopathogenic fungi Magnaporthe grisea (50% inhibitory concentration [IC50] = 5 microg/ml) and Botrytis cinerea (IC50 = 20 microg/ml) but is weakly active against the phytopathogenic bacterium Pseudomonas cichorii. This virus-mediated expression system is a rapid and efficient method to produce and characterize antimicrobial proteins in plants. It is particularly useful for the study of proteins that are difficult to produce with other expression systems.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plantas Tóxicas
/
Nicotiana
/
Potexvirus
/
Defensinas
/
Vetores Genéticos
/
Anti-Infecciosos
Idioma:
En
Revista:
Mol Plant Microbe Interact
Assunto da revista:
BIOLOGIA MOLECULAR
/
BOTANICA
/
MICROBIOLOGIA
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Japão