A cytotoxicity assay for the detection and differentiation of two families of shellfish toxins.
Toxicon
; 39(7): 1021-7, 2001 Jul.
Article
em En
| MEDLINE
| ID: mdl-11223091
ABSTRACT
There is an urgent need for an alternative to the mouse bioassay for the detection of algal toxins in shellfish on both analytical and animal welfare grounds. Several alternative methodologies have been described, but have not gained widespread acceptance to date, because each assay measures only one or a small number of related phycotoxins out of the increasing range that needs to be detected. A simple cytotoxicity assay using either the HepG2 or ECV-304 cell lines is described with two end-point measurements, which can detect and distinguish between two unrelated classes of phycotoxins. Morphological examination following 3h exposure to the sample enables the detection of the diarrhetic shellfish poisons, including okadaic acid and related toxins. Viability testing using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide), following 24h exposure of the same cells to the sample, reveals a second class of toxin, which is most probably the newly-described toxin, azaspiracid. This assay should play an important role in shellfish monitoring in the future.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Frutos do Mar
/
Toxinas Marinhas
/
Antineoplásicos
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Toxicon
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Irlanda