[Construction of peptide mimetics of an epitope of the human immunodeficiency virus (HIV-1) gp41 protein, recognized by virus-neutralizing antibodies 2F5]. / Polucheni peptidov-imitatorov épitopa belka gp41 virusa immunodefitsita cheloveka (VICh-1) uznavaemogo virusneitralizuiushchimi antitelami 2F5.
Mol Biol (Mosk)
; 35(1): 146-51, 2001.
Article
em Ru
| MEDLINE
| ID: mdl-11234374
ABSTRACT
A phase peptide library was screened with virus-neutralizing monoclonal antibodies (MCA) 2F5 which recognize a conserved epitope of HIV-1 gp41. Phages that expose peptides specifically binding with MCA 2F5 were selected by ELISA. Amino acid sequence analysis revealed a homology to region 662-671 of HIV-1 HB10 gp160 for most peptides. The major role in recognition was ascribed to Asp-664, Lys-665, and Trp-666. The epitope-mimicking peptides were tested for immunogenicity. Antibodies to gp41 were detected in serum of immunized rabbits.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Proteína gp41 do Envelope de HIV
/
HIV-1
/
Mimetismo Molecular
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Anticorpos Monoclonais
/
Epitopos
Limite:
Animals
Idioma:
Ru
Revista:
Mol Biol (Mosk)
Ano de publicação:
2001
Tipo de documento:
Article
País de publicação:
FEDERAÇÃO RUSSA
/
RU
/
RUSIA
/
RUSSIA