Characterization of immunoreactive acetyl-Ser-Asp-Lys-Pro in human plasma and urine by liquid chromatography-electrospray mass spectrometry.
J Chromatogr B Biomed Sci Appl
; 752(1): 69-75, 2001 Mar 05.
Article
em En
| MEDLINE
| ID: mdl-11254199
ABSTRACT
The tetrapeptide AcSDKP, a natural and specific substrate of angiotensin I-converting enzyme (ACE), is a negative regulator of hematopoiesis. AcSDKP has been measured in various biological media using an enzyme immunoassay (EIA), but its presence in human plasma and urine has not been formally established. By using immunoaffinity extraction and liquid chromatography-electrospray mass spectrometry, we demonstrate that AcSDKP-like immunoreactivity measured with EIA in plasma and urine samples from untreated, captopril- (an ACE inhibitor) and AcSDKP-treated subjects corresponds to AcSDKP. The present study confirms that AcSDKP is naturally present in human plasma and urine and that EIA is reliable for its measurement in such media.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oligopeptídeos
/
Cromatografia de Afinidade
Limite:
Humans
Idioma:
En
Revista:
J Chromatogr B Biomed Sci Appl
Assunto da revista:
QUIMICA CLINICA
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
França