Crystallization and preliminary X-ray diffraction studies of the guanylate kinase-like domain of PSD-95 protein from rat.
Acta Crystallogr D Biol Crystallogr
; 57(Pt 4): 616-7, 2001 Apr.
Article
em En
| MEDLINE
| ID: mdl-11264600
The PSD-95 (postsynaptic density-95) protein, one of the members of the MAGUK (membrane-associated guanylate kinase) family, is composed of three PDZ domains, one SH3 domain and one guanylate kinase-like (GK) domain. The GK domain mediates the scaffolding function of PSD-95 by protein--protein interaction. Here, the GK domain was subcloned, expressed as an intein fusion protein, purified without the intein and then crystallized at room temperature by the hanging-drop vapour-diffusion method using PEG 8000 as a precipitant. The complete native data set was collected to a resolution of 2.35 A using flash-cooling. The crystals belong to the primitive tetragonal space group P4(3) (or P4(1)), with unit-cell parameters a = b = 70.03 (4), c = 37.64 (1) A.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Núcleosídeo-Fosfato Quinase
/
Proteínas do Tecido Nervoso
Limite:
Animals
Idioma:
En
Revista:
Acta Crystallogr D Biol Crystallogr
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Coréia do Sul
País de publicação:
Estados Unidos