Three-dimensional localisation of fluorescence resonance energy transfer in living cells under two-photon excitation.
Scanning
; 23(1): 9-13, 2001.
Article
em En
| MEDLINE
| ID: mdl-11272338
ABSTRACT
Three-dimensional (3-D) imaging of fluorescence resonance energy transfer (FRET) in human cells under two-photon excitation was demonstrated in this study. A sample was prepared by expressing a donor and an acceptor in living cells and using an antibody to secure the proximity of contact between the donor and the acceptor. The quenching of fluorescence emission of a donor in the double-labelled cells indicates the presence of FRET that occurred in these living cells. Because of the quadratic relation of the excitation power, 3-D localisation of FRET becomes possible.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Fluorescência
/
Microscopia Confocal
Limite:
Animals
Idioma:
En
Revista:
Scanning
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Austrália