Increased nitric oxide synthase activity in a model of serotonin depletion.
Brain Res Bull
; 54(2): 199-205, 2001 Jan 15.
Article
em En
| MEDLINE
| ID: mdl-11275409
ABSTRACT
Serotonin (5HT) containing cell bodies are localized in mesencephalic and rhombencephalic raphe nuclei. It has been proposed that 5HT could be involved in neuronal development and plasticity. In the central nervous system, nitric oxide (NO) has been postulated as a neurotransmitter and neuromodulator, and has been implicated in neurotoxicity as well as in neuroprotection. Using the nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) technique, NO synthesizing neurons were described in raphe nuclei. By immunohistochemistry, nitric oxide synthase (NOS) was found colocalized with 5HT in some dorsal raphe nucleus (DRN) neurons. In a model of inhibition of 5HT synthesis produced by daily administration of parachlorophenilalanine during 14 days, we have studied the relationship between 5HT and NO systems after 5HT depletion by histochemical and immunocytochemical methods. After the treatment, we observed an important reduction of 5HT immunostaining in the DRN and enhanced NOS activity demonstrated by NADPH-d technique, especially in the dorsomedial and ventromedial subgroups. In spite of the increased NOS activity, we could not observe significant changes in the NOS-immunoreactivity in the DRN after 5HT depletion. These results could indicate that 5HT depletion is concomitant with changes in NOS activity without affecting NOS expression in the DRN.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Núcleos da Rafe
/
Serotonina
/
Óxido Nítrico Sintase
/
Neurônios
Limite:
Animals
Idioma:
En
Revista:
Brain Res Bull
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Argentina