Ouabain-sensitive and insensitive acetylcholine receptors on the membrane of the same neuron in Helix pomatia.
Comp Biochem Physiol C Toxicol Pharmacol
; 128(4): 511-20, 2001 Apr.
Article
em En
| MEDLINE
| ID: mdl-11301293
ABSTRACT
Using internally dialyzed neurons of Helix pomatia as a model, the effect of structural analogs of acetylcholine (ACh) were investigated for their cholinomimetic properties on A- and B-types of ACh-responses. Specifically we analyzed choline esters of N-para- and ortho-alkoxybenzoyl-beta-alanines, (CH(3)O-, C(2)H(5)O-, C(3)H(7)O-, iso-C(3)H(7)O-, C(4)H(9)O-, iso-C(4)H(9)O-, C(5)H(11)O-, iso-C(5)H(11)O-), (in all, 16 combinations). The compounds evoked differing sensitivities of response to factors de-activating the Na-K-pump (ouabain and K-free solution). Most compounds resembled ACh ionic currents caused by these compounds were inhibited by Na-K pump blockers in the case of A-type responses - B-type responses were insensitive to these factors. Ionic currents induced by choline esters of p-, o-propoxy- and iso-propoxybenzoyl-beta-alanines were insensitive to ouabain and K-free solution in the case of A- and B-type responses. Ionic currents induced by the choline ester of p-butoxybenzoyl-beta-alanine were inhibited by ouabain and K-free solution on both types of neuron. The results allow us to classify choline esters of p-, o-propoxy- and iso-propoxybenzoyl-beta-alanines as N-cholinomimetics, while the choline ester of p-butoxybenzoyl-beta-alanine can be considered as M-cholinomimetic. We conclude that both M- and N-type ACh receptors exist on the membrane of the same neurons.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ouabaína
/
Receptores Colinérgicos
/
Neurônios
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Comp Biochem Physiol C Toxicol Pharmacol
Assunto da revista:
FARMACOLOGIA
/
TOXICOLOGIA
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Armênia