Use of DNA-based diagnostic methods for human leishmaniasis in Minas Gerais, Brazil.
Acta Trop
; 78(3): 261-7, 2001 Mar 30.
Article
em En
| MEDLINE
| ID: mdl-11311189
ABSTRACT
DNA hybridisation was used to type 26 samples from lesions of human patients from the Rio Doce Valley (Minas Gerais, Brazil) clinically diagnosed as having cutaneous leishmaniasis, using kinetoplast DNA (kDNA) cloned mini-circle probes specific for the Leishmania mexicana and Leishmania braziliensis complexes. All samples were found to belong to the L. braziliensis complex. When biopsies were pressed directly onto touch blot membranes 38.5% of the samples were positive. The positivity and specificity obtained were both 100% when cultured blotted parasites were used. The results were confirmed by polymerase chain reaction (PCR) analysis using primers specific for the L. mexicana and L. braziliensis complexes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Leishmania braziliensis
/
Leishmania mexicana
/
DNA de Protozoário
/
Leishmaniose Cutânea
/
DNA de Cinetoplasto
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Humans
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Acta Trop
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Brasil