Continuous spectrophotometric assay for beta-glucuronidase.
Biotechniques
; 30(4): 846-50, 2001 Apr.
Article
em En
| MEDLINE
| ID: mdl-11314267
ABSTRACT
A continuous spectrophotometric assay has been developed for detecting beta-glucuronidase activity. In the assay, Para-nitrophenyl beta-D-glucuronide is cleaved to yield a chromophoric product. With the commercial E. coli enzyme, it is demonstrated that the reactions can be continuously monitored by the increase of absorbance at 405 nm. The method is highly sensitive and able to detect less than 1.4 x 10(-4) U/mL of the enzyme activity in solution. Such a new assay offers significant advantages over the existing discontinuous methods and should be useful for both routine enzyme assay and accurate kinetic studies.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espectrofotometria
/
Glucuronidase
Idioma:
En
Revista:
Biotechniques
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Canadá