Addition of substrate-binding domains increases substrate-binding capacity and specific activity of a chitinase from Trichoderma harzianum.
FEMS Microbiol Lett
; 198(1): 57-63, 2001 Apr 20.
Article
em En
| MEDLINE
| ID: mdl-11325554
ABSTRACT
Chitinase Chit42 from Trichoderma harzianum CECT 2413 is considered to play an important role in the biocontrol activity of this fungus against plant pathogens. Chit42 lacks a chitin-binding domain (ChBD). We have produced hybrid chitinases with stronger chitin-binding capacity by fusing to Chit42 a ChBD from Nicotiana tabacum ChiA chitinase and the cellulose-binding domain from cellobiohydrolase II of Trichoderma reesei. The chimeric chitinases had similar activities towards soluble substrate but higher hydrolytic activity than the native chitinase on high molecular mass insoluble substrates such as ground chitin or chitin-rich fungal cell walls.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Trichoderma
/
Quitina
/
Quitinases
Idioma:
En
Revista:
FEMS Microbiol Lett
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Espanha