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Addition of substrate-binding domains increases substrate-binding capacity and specific activity of a chitinase from Trichoderma harzianum.
Limón, M C; Margolles-Clark, E; Benítez, T; Penttilä, M.
Afiliação
  • Limón MC; Departamento de Genética Facultad de Biologia, Universidad de Servilla, Apartado 1095, E-41080 Servilla, Spain.
FEMS Microbiol Lett ; 198(1): 57-63, 2001 Apr 20.
Article em En | MEDLINE | ID: mdl-11325554
ABSTRACT
Chitinase Chit42 from Trichoderma harzianum CECT 2413 is considered to play an important role in the biocontrol activity of this fungus against plant pathogens. Chit42 lacks a chitin-binding domain (ChBD). We have produced hybrid chitinases with stronger chitin-binding capacity by fusing to Chit42 a ChBD from Nicotiana tabacum ChiA chitinase and the cellulose-binding domain from cellobiohydrolase II of Trichoderma reesei. The chimeric chitinases had similar activities towards soluble substrate but higher hydrolytic activity than the native chitinase on high molecular mass insoluble substrates such as ground chitin or chitin-rich fungal cell walls.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Trichoderma / Quitina / Quitinases Idioma: En Revista: FEMS Microbiol Lett Ano de publicação: 2001 Tipo de documento: Article País de afiliação: Espanha
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Trichoderma / Quitina / Quitinases Idioma: En Revista: FEMS Microbiol Lett Ano de publicação: 2001 Tipo de documento: Article País de afiliação: Espanha