Specific identification of Lactobacillus helveticus by PCR with pepC, pepN and htrA targeted primers.
FEMS Microbiol Lett
; 198(1): 85-9, 2001 Apr 20.
Article
em En
| MEDLINE
| ID: mdl-11325558
ABSTRACT
Specific regions in three genes coding for aminopeptidases C and N, and a trypsin-like serine protease were selected as species-specific primer sequences for rapid and reliable identification of Lactobacillus helveticus strains. The PCR procedures carried out gave specific 524-, 726- and 918-bp amplificates, with DNA isolated from L. helveticus. No PCR product was generated for closely related bacteria. The amplification products were also screened for their species specificity in dot blot hybridization with representatives of the most closely related genera and species and a number of other bacterial species.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Serina Endopeptidases
/
Reação em Cadeia da Polimerase
/
Proteínas Periplásmicas
/
Aminopeptidases
/
Proteínas de Choque Térmico
/
Lactobacillus
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
FEMS Microbiol Lett
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Itália