Ssb1 chaperone is a [PSI+] prion-curing factor.
Curr Genet
; 39(2): 62-7, 2001 Apr.
Article
em En
| MEDLINE
| ID: mdl-11405097
ABSTRACT
Yeast SUP7' or SUP11 nonsense suppressors have no phenotypic expression in strains deficient in the isopentenylation of A37 in tRNA. Here we show that such strains spontaneously produce cells with a nonsense suppressor phenotype which is related to the cytoplasmically inherited determinant and manifests all the key features of the [PSI+] prion. A screen of a multicopy yeast genomic library for genes that inactivate the [PSI+]-related suppressor phenotype resulted in the isolation of the SSB1 gene. Moreover, we demonstrate that multicopy plasmid encoding the Ssb1 chaperone cures cells of the [PSI+] prion.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Príons
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Proteínas Fúngicas
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Chaperonas Moleculares
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Proteínas de Saccharomyces cerevisiae
Limite:
Animals
Idioma:
En
Revista:
Curr Genet
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Polônia
País de publicação:
EEUU
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ESTADOS UNIDOS
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ESTADOS UNIDOS DA AMERICA
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EUA
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UNITED STATES
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UNITED STATES OF AMERICA
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US
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USA