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Beta2-adrenergic receptor/cyclic adenosine monophosphate (cAMP) leads to JNK activation through Rho family small GTPases.
Yamauchi, J; Hirasawa, A; Miyamoto, Y; Itoh, H; Tsujimoto, G.
Afiliação
  • Yamauchi J; Department of Molecular Cell Pharmacology, National Children's Medical Research Center, 3-35-31 Taishido, Tokyo, Setagaya-ku, 154-8509, Japan.
Biochem Biophys Res Commun ; 284(5): 1199-203, 2001 Jun 29.
Article em En | MEDLINE | ID: mdl-11414711
Gi- and Gq-coupled G protein-coupled receptors (GPCRs) have been shown to activate c-Jun N-terminal kinase (JNK), a subfamily of mitogen-activated protein kinases (MAPKs), through Rho family small GTPases in mammalian cells. We investigated the signaling pathway linking the Gs-coupled beta2-adrenergic receptor with JNK, using smooth muscle DDT1 MF-2 cells, which natively express the beta2-adrenergic receptor. Stimulation of the beta2-adrenergic receptor activated JNK in a time-dependent manner, and a cell-permeable cyclic adenosine monophosphate analogue (8-Br-cAMP) activated JNK. The beta2-adrenergic receptor- or 8-Br-cAMP-induced activation of JNK required Rho family small GTPases. Also, the beta2-adrenergic receptor or 8-Br-cAMP induced activation of Rho family small GTPases. These results demonstrate that the beta2-adrenergic receptor/cAMP leads to JNK activation through Rho family small GTPases in DDT1 MF-2 cells. Activation of Rho family small GTPases may provide a common step in GPCR-mediated JNK activation.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Receptores Adrenérgicos beta 2 / AMP Cíclico / Proteínas rho de Ligação ao GTP / Proteínas Quinases Ativadas por Mitógeno Limite: Animals / Humans Idioma: En Revista: Biochem Biophys Res Commun Ano de publicação: 2001 Tipo de documento: Article País de afiliação: Japão País de publicação: Estados Unidos
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Receptores Adrenérgicos beta 2 / AMP Cíclico / Proteínas rho de Ligação ao GTP / Proteínas Quinases Ativadas por Mitógeno Limite: Animals / Humans Idioma: En Revista: Biochem Biophys Res Commun Ano de publicação: 2001 Tipo de documento: Article País de afiliação: Japão País de publicação: Estados Unidos