Extensive traumatic soft tissue loss: reconstruction in severely injured patients using cultured hyaluronan-based three-dimensional dermal and epidermal autografts.
J Trauma
; 50(6): 1125-36, 2001 Jun.
Article
em En
| MEDLINE
| ID: mdl-11426129
ABSTRACT
BACKGROUND:
This report demonstrates the potential of two-stage autologous keratodermal grafting as a starting point for noninvasive reconstruction of extensive traumatic soft tissue defects.METHODS:
In three severely injured patients, skin biopsies for cell cultivation were taken. Cultured "neodermis" consisting of cultured autologous fibroblasts grown on biocompatible three-dimensional scaffolds made up of benzyl ester of hyaluronan was grafted on conditioned defect areas. After ingrowth of dermal substitutes, transplantation of cultured autologous keratinocytes on hyaluronan-based laser-perforated membranes was performed. Ten days later, a 0.2-mm thin, 16 meshed autograft was overlaid. Clinical follow-up, histologic, and immunohistochemical findings were documented.RESULTS:
Grafting with cultured autologous fibroblasts revealed a suitable dermal tissue replacement. Epithelialization was evident after transplantation of keratinocytes. Final closure of the defects with "normoelastic" tissue properties was achieved after thin mesh-grafting.CONCLUSION:
Preliminary findings with the described method seem to be very promising. As in all fields of tissue engineering, long-term studies and further follow-up are required.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Adjuvantes Imunológicos
/
Transplante de Pele
/
Lesões dos Tecidos Moles
/
Ácido Hialurônico
Limite:
Adult
/
Aged
/
Child
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
J Trauma
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Alemanha