Identification of coding single-nucleotide polymorphisms in human taste receptor genes involving bitter tasting.
Biochem Biophys Res Commun
; 285(1): 147-51, 2001 Jul 06.
Article
em En
| MEDLINE
| ID: mdl-11437385
ABSTRACT
T2Rs comprise a G-protein-coupled receptor superfamily that contains functionally defined bitter taste receptors. Here we report the tissue expressions and coding single-nucleotide polymorphisms (cSNPs) in human T2R genes (hT2R3, hT2R4, and hT2R5) on chromosome 7q31. We first demonstrated that hT2R3, hT2R4, and hT2R5 are actually expressed in the circumvallate papillae of the human tongue by reverse transcription-polymerase chain reaction (RT-PCR). We identified six cSNPs within the T2R receptor genes. The hT2R4 and hT2R5 contained four and one cSNPs that cause missense mutations, respectively, while hT2R3 included one silent nucleotide mutation. However, we could not find any nonsense mutations that resulted in a frameshift or a premature stop codon within the open reading frames. Genotype frequencies of each cSNP were in Hardy-Weinberg equilibrium. The identification of nucleotide diversity and amino acid polymorphisms in human T2R receptors could help clarify individual differences in the acceptability and sensitivity to bitter compounds.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Papilas Gustativas
/
Receptores de Superfície Celular
/
Polimorfismo de Nucleotídeo Único
/
Receptores Acoplados a Proteínas G
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Japão