Mismatch repair detection (MRD): high-throughput scanning for DNA variations.
Hum Mol Genet
; 10(16): 1657-64, 2001 Aug 01.
Article
em En
| MEDLINE
| ID: mdl-11487569
ABSTRACT
Although there are several methods for genotyping previously identified single nucleotide polymorphisms (SNPs), there is a paucity of approaches for high-throughput scanning for unknown variations. Mismatch repair detection (MRD) utilizes a bacterial mismatch repair system in vivo to detect sequence variants in human DNA samples. We describe modifications in MRD that allow a high degree of parallel processing, and use this modified version to accurately scan for variations in 35 different human DNA fragments simultaneously. MRD's potential for high-throughput scanning can be used to identify new SNPs and to comprehensively compare sequences between patients and controls for identifying disease susceptibility alleles.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pareamento Incorreto de Bases
/
Reparo do DNA
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Humans
Idioma:
En
Revista:
Hum Mol Genet
Assunto da revista:
BIOLOGIA MOLECULAR
/
GENETICA MEDICA
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Estados Unidos