Mouse B1 cell line responds to lipopolysaccharide via membrane-bound CD14.
J Endotoxin Res
; 7(1): 39-43, 2001.
Article
em En
| MEDLINE
| ID: mdl-11521080
ABSTRACT
The role of membrane-bound CD14 in the response of mouse B1 cell lines to lipopolysaccharide (LPS) was studied. The surface profile of mouse TH2.52 B cells was positive for CD5, IgM, B220, CD11b and F4/80, suggesting that TH2.52 cells carried the typical phenotype of B1 cells. Furthermore, TH2.52 B1 cells were found to express membrane-bound CD14, which plays a critical role in LPS recognition. TH2.52 B1 cells responded to a very low concentration of LPS and exhibited (i) augmentation of DNA synthesis; (ii) activation of nuclear factor (NF)-kappaB; and (iii) phosphorylation of extracellular signal regulated kinase 1/2 (Erk1/2). They were markedly inhibited by anti-CD14 antibody. Therefore, the expression of membrane-bound CD14 was suggested to provide high sensitivity to LPS for TH2.52 B1 cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Membrana Celular
/
Lipopolissacarídeos
/
Receptores de Lipopolissacarídeos
Limite:
Animals
Idioma:
En
Revista:
J Endotoxin Res
Assunto da revista:
ANGIOLOGIA
/
BACTERIOLOGIA
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Japão