Your browser doesn't support javascript.
loading
Differential expression of cell-wall-related genes during the formation of tracheary elements in the Zinnia mesophyll cell system.
Milioni, D; Sado, P E; Stacey, N J; Domingo, C; Roberts, K; McCann, M C.
Afiliação
  • Milioni D; Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park, Colney, UK.
Plant Mol Biol ; 47(1-2): 221-38, 2001 Sep.
Article em En | MEDLINE | ID: mdl-11554474
Plants, animals and some fungi undergo processes of cell specialization such that specific groups of cells are adapted to carry out particular functions. One of the more remarkable examples of cellular development in higher plants is the formation of water-conducting cells that are capable of supporting a column of water from the roots to tens of metres in the air for some trees. The Zinnia mesophyll cell system is a remarkable tool with which to study this entire developmental pathway in vitro. We have recently applied an RNA fingerprinting technology, to allow the detection of DNA fragments derived from RNA using cDNA synthesis and subsequent PCR-amplified fragment length polymorphisms (cDNA-AFLP), to systematically characterize hundreds of the genes involved in the process of tracheary element formation. Building hoops of secondary wall material is the key structural event in forming functional tracheary elements and we have identified over 50 partial sequences related to cell walls out of 600 differentially expressed cDNA fragments. The Zinnia system is an engine of gene discovery which is allowing us to identify and characterize candidate genes involved in cell wall biosynthesis and assembly.
Assuntos
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plantas / Parede Celular / Folhas de Planta Tipo de estudo: Prognostic_studies Idioma: En Revista: Plant Mol Biol Assunto da revista: BIOLOGIA MOLECULAR / BOTANICA Ano de publicação: 2001 Tipo de documento: Article País de publicação: Holanda
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plantas / Parede Celular / Folhas de Planta Tipo de estudo: Prognostic_studies Idioma: En Revista: Plant Mol Biol Assunto da revista: BIOLOGIA MOLECULAR / BOTANICA Ano de publicação: 2001 Tipo de documento: Article País de publicação: Holanda