Establishment and characterization of a cell line, MT-213-VGH, isolated from a mixed müllerian tumor of the uterus.
Acta Cytol
; 45(5): 683-90, 2001.
Article
em En
| MEDLINE
| ID: mdl-11575644
ABSTRACT
OBJECTIVE:
To establish a cell line from a woman with malignant mixed müllerian tumor of the uterus and to examine the biologic properties of this cell line (MT-213-VGH). STUDYDESIGN:
Cells were cloned by the limiting dilution method. Histologic staining of mixed müllerian (mesodermal) tumor (MMMT) cells was performed with May-Grünwald-Giemsa and hematoxylin and eosin stain. After more than 20 passages, cells were used to estimate the population-doubling time and colony-forming efficiency of MMMT cells. The cell line exhibited considerable variation in the degree of sensitivity to diverse chemotherapy drugs in vitro.RESULTS:
MMMT cells containing antigens for vimentin and myoglobin were detected, but those for CA-125, carcinoembryonic antigen, cytoskeleton, desmin, epithelial membrane antigen and fibronectin were not found. In addition, MT-213-VGH cells contained a mucinous substance; its chromosome model number is 45. This cell line showed differential sensitivities to chemotherapeutic agents, such as bleomycin, cisplatin, 5-fluorouracil and vinblastine.CONCLUSION:
The establishment and availability of the number cell line MT-213-VGH for a malignant mixed müllerian tumor of the uterus should assist in research on new methods of managing this type of gynecologic cancer.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Uterinas
/
Células Tumorais Cultivadas
/
Tumor Mulleriano Misto
Limite:
Female
/
Humans
/
Middle aged
Idioma:
En
Revista:
Acta Cytol
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Taiwan