T cell-dependent inducible nitric oxide synthase production and ultrastructural morphology in BALB/c mice infected with Mycobacterium avium subspecies paratuberculosis.
J Comp Pathol
; 125(2-3): 137-44, 2001.
Article
em En
| MEDLINE
| ID: mdl-11578129
ABSTRACT
Euthymic BALB/c and athymic nude BALB/c mice aged 3-8 days were infected intraperitoneally with Mycobacterium avium subspecies paratuberculosis (ATCC strain 19698). After euthanasia at 5 months post-inoculation, hepatic granulomas were evaluated by morphometric analysis of digital images captured from light microscopy sections, by electron microscopy and by immunohistochemical methods. Euthymic mice differed from athymic mice in that (1) their hepatic granulomas were smaller, contained fewer bacteria, and produced more inducible nitric oxide synthase, and (2) their hepatic macrophages contained fewer bacteria, a higher percentage of degraded bacteria, and increased numbers of primary lysosomes. The study showed that macrophage activation was markedly less in the T cell-deficient athymic mice than in the euthymic mice.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Paratuberculose
/
Linfócitos T
/
Mycobacterium avium subsp. paratuberculosis
/
Óxido Nítrico Sintase
Limite:
Animals
Idioma:
En
Revista:
J Comp Pathol
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Estados Unidos