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The transgenic ICP4 promoter is activated in Schwann cells in trigeminal ganglia of mice latently infected with herpes simplex virus type 1.
Taus, N S; Mitchell, W J.
Afiliação
  • Taus NS; Department of Veterinary Pathobiology, University of Missouri, Columbia, Missouri 65211, USA.
J Virol ; 75(21): 10401-8, 2001 Nov.
Article em En | MEDLINE | ID: mdl-11581408
Herpes simplex virus type 1 (HSV-1) establishes a latent infection in neurons of sensory ganglia, including those of the trigeminal ganglia. Latent viral infection has been hypothesized to be regulated by restriction of viral immediate-early gene expression in neurons. Numerous in situ hybridization studies in mice and in humans have shown that transcription from the HSV-1 genome in latently infected neurons is limited to the latency-associated transcripts. In other studies, immediate-early gene (ICP4) transcripts have been detected by reverse transcription-PCR (RT-PCR) in homogenates of latently infected trigeminal ganglia of mice. We used reporter transgenic mice containing the HSV-1(F) ICP4 promoter fused to the coding sequence of the beta-galactosidase gene to determine whether neurons in latently infected trigeminal ganglia activated the ICP4 promoter. Mice were inoculated via the corneal route with HSV-1(F). At 5, 11, 23, and 37 days postinfection (dpi), trigeminal ganglia were examined for beta-galactosidase-positive cells. The numbers of beta-galactosidase-positive neurons and nonneuronal cells were similar at 5 dpi. The number of positive neurons decreased at 11 dpi and returned to the level of mock-inoculated transgenic controls at 23 and 37 dpi. The number of positive nonneuronal cells increased at 11 and 23 dpi and remained elevated at 37 dpi. Viral proteins were detected in neurons and nonneuronal cells in acutely infected ganglia, but were not detected in latently infected ganglia. Colabeling experiments confirmed that the transgenic ICP4 promoter was activated in Schwann cells during latent infection. These findings suggest that the cells that express the HSV-1 ICP4 gene in latently infected ganglia are not neurons.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células de Schwann / Gânglio Trigeminal / Regiões Promotoras Genéticas / Proteínas Imediatamente Precoces / Herpesvirus Humano 1 / Herpes Simples Limite: Animals Idioma: En Revista: J Virol Ano de publicação: 2001 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células de Schwann / Gânglio Trigeminal / Regiões Promotoras Genéticas / Proteínas Imediatamente Precoces / Herpesvirus Humano 1 / Herpes Simples Limite: Animals Idioma: En Revista: J Virol Ano de publicação: 2001 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Estados Unidos