High-performance liquid chromatographic assay for abacavir and its two major metabolites in human urine and cerebrospinal fluid.
J Chromatogr B Biomed Sci Appl
; 762(2): 165-73, 2001 Oct 25.
Article
em En
| MEDLINE
| ID: mdl-11678376
ABSTRACT
A simple, reversed-phase HPLC assay has been developed and validated to measure the HIV-1 reverse transcriptase inhibitor abacavir and its two major metabolites, a 5'-glucuronide and a 5'-carboxylate, in human urine and cerebrospinal fluid. Sample preparation involved centrifuging to minimize particulates, then diluting the supernatant before HPLC separation and ultraviolet detection at 295 nm. The method described was used successfully to measure concentrations of abacavir and its two major metabolites in urine and cerebrospinal fluid from HIV-1 infected subjects.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Didesoxinucleosídeos
/
Infecções por HIV
/
Cromatografia Líquida de Alta Pressão
/
Inibidores da Transcriptase Reversa
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
J Chromatogr B Biomed Sci Appl
Assunto da revista:
QUIMICA CLINICA
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Estados Unidos