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PCR analyses of tRNA intergenic spacer, 16S-23S internal transcribed spacer, and randomly amplified polymorphic DNA reveal inter- and intraspecific relationships of Enterobacter cloacae strains.
Clementino, M M; de Filippis, I; Nascimento, C R; Branquinho, R; Rocha, C L; Martins, O B.
Afiliação
  • Clementino MM; Department of Microbiology, National Institute of Quality Control in Health, FIOCRUZ, Rio de Janeiro, Brazil. maysa@alpha.incqs.fiocruz.br
J Clin Microbiol ; 39(11): 3865-70, 2001 Nov.
Article em En | MEDLINE | ID: mdl-11682499
ABSTRACT
PCR analysis of tRNA intergenic spacer (tDNA-PCR) and of the 16S-23S internal transcribed spacer (ITS-PCR) and random amplified polymorphic DNA (RAPD) analysis were evaluated for their usefulness in characterization of Enterobacter cloacae strains isolated from both clinical origins and vaccine microbial contamination. tDNA-PCR presented specific and reproducible patterns for Enterobacter sakazakii ATCC 29004, Enterobacter aerogenes ATCC 13048, and Enterobacter cloacae ATCC 13047 and 23355 that presented the same profile for all 16 E. cloacae isolates, offering an alternative tool for species-level identification. ITS-PCR and RAPD analysis yielded completely different banding patterns for the 20 strains studied, except for E. cloacae strains isolated from different batches of vaccine that exhibited a unique pattern, suggesting contamination by the same strain. The combined use of tDNA-PCR and ITS-PCR in a one-step protocol allows accurate identification and typing of E. cloacae strains a few hours after the colony has been isolated.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Reação em Cadeia da Polimerase / Enterobacter cloacae / Ribotipagem Tipo de estudo: Clinical_trials / Evaluation_studies / Guideline / Prognostic_studies Limite: Humans Idioma: En Revista: J Clin Microbiol Ano de publicação: 2001 Tipo de documento: Article País de afiliação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Reação em Cadeia da Polimerase / Enterobacter cloacae / Ribotipagem Tipo de estudo: Clinical_trials / Evaluation_studies / Guideline / Prognostic_studies Limite: Humans Idioma: En Revista: J Clin Microbiol Ano de publicação: 2001 Tipo de documento: Article País de afiliação: Brasil