Thyroid hormone regulates TAG-1 expression in the developing rat brain.
Eur J Neurosci
; 14(8): 1209-18, 2001 Oct.
Article
em En
| MEDLINE
| ID: mdl-11703450
ABSTRACT
TAG-1 is a member of the immunoglobulin superfamily of cell adhesion molecules thought to play important roles in neuronal differentiation and the establishment of connectivity during brain development. Because these are processes also affected by hypothyroidism, we studied the effects of thyroid hormone deprivation and administration on TAG-1 expression in the developing rat brain. By in situ hybridization, immunohistochemistry and Western blotting we found that TAG-1 RNA and protein levels are upregulated in the hypothyroid brain. From embryonic day 20 to postnatal day (P) 15, elevated TAG-1 RNA was found in several areas including the cerebral cortex, hippocampus and olfactory bulb. In agreement with this, TAG-1 protein was overexpressed in the major fibre tracts arising from these structures, including the corpus callosum, anterior and hippocampal commissures and lateral olfactory tract. A similar overexpression of TAG-1 by hypothyroidism was detected in the cerebellum, but starting only at P15. In all cases, elevation of TAG-1 RNA and protein expression could be reversed by thyroid hormone treatment. These results show that the deregulation of TAG-1 might contribute to the alterations caused by the lack of thyroid hormone during brain development.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Axônios
/
Tri-Iodotironina
/
Encéfalo
/
Glicoproteínas de Membrana
/
Moléculas de Adesão Celular Neuronais
/
Regulação para Cima
/
Regulação da Expressão Gênica no Desenvolvimento
/
Hipotireoidismo
Limite:
Animals
Idioma:
En
Revista:
Eur J Neurosci
Assunto da revista:
NEUROLOGIA
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Espanha