RBC storage for 11 weeks.
Transfusion
; 41(12): 1586-90, 2001 Dec.
Article
em En
| MEDLINE
| ID: mdl-11778076
ABSTRACT
BACKGROUND:
Increasing the length of RBC storage can increase both RBC availability and quality. This work addresses 11-week RBC storage in experimental ASs (EASs). STUDY DESIGN ANDMETHODS:
Three studies were performed. In the first, 24-hour in vivo recovery of (51)Cr-labeled autologous RBCs was measured in nine volunteers after storage of their RBCs for 11 weeks in EAS 67. In the second study, 4 units of blood were divided and stored in aliquots with an EAS containing 0, 15, 30, or 45 mmol per L of mannitol; then hemolysis, RBC morphology, and microvesicle protein were measured. In the third study, 6 full units were stored for 12 weeks in the EAS containing 30 mmol per L of mannitol, with weekly sampling for morphologic and biochemical measures of RBC quality.RESULTS:
RBCs stored for 11 weeks in EAS-67 had a mean 24-hour in vivo recovery of 79 +/- 5 percent, but the hemolysis was 1.35 +/- 0.68 percent. Increasing mannitol content of the EAS reduced hemolysis but increased microvesiculation. EAS-76, with 30 mmol per L of mannitol allowed 11-week storage with 0.48 +/- 0.10 percent hemolysis at 11 weeks and 0.62 +/- 0.14 percent hemolysis at 12 weeks.CONCLUSION:
It is possible to store RBCs for 11 weeks in EAS with greater than 75 percent recovery and less than 1 percent hemolysis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Preservação de Sangue
/
Eritrócitos
Tipo de estudo:
Evaluation_studies
Limite:
Adult
/
Humans
Idioma:
En
Revista:
Transfusion
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Estados Unidos