Targeting vectors for gene diversification by meiotic recombination in Neurospora crassa.
Plasmid
; 47(1): 18-25, 2002 Jan.
Article
em En
| MEDLINE
| ID: mdl-11798282
ABSTRACT
We have constructed a pair of vectors, pDV2 and pDV3, that enable targeted insertion of exogenous DNA into Linkage Group I of Neurospora crassa at the his-3 locus. Transplaced sequences are inserted between his-3 and the cog(L) recombination hot spot and include his-3 mutations that allow meiotic recombination initiated by cog(L) to be monitored. Selection of correctly placed transforming DNA is based on complementation between different his-3 alleles borne by the plasmids and transformation hosts. The system allows investigation of the effect of any given sequence on recombination as well as diversification of sets of related sequences in vivo for directed evolution of genes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Recombinação Genética
/
Vetores Genéticos
/
Meiose
/
Neurospora crassa
Idioma:
En
Revista:
Plasmid
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Austrália