Ex-vivo short-term culture and developmental assessment of Plasmodium vivax.
Trans R Soc Trop Med Hyg
; 95(6): 677-80, 2001.
Article
em En
| MEDLINE
| ID: mdl-11816444
ABSTRACT
A simple reproducible method for short-term ex-vivo Plasmodium vivax culture is presented in which glucose, ascorbic acid, thiamine, hypoxanthine, and 50% human AB+ serum are added to the standard P. falciparum in-vitro culture medium. Culture of freshly obtained blood samples from patients with acute vivax malaria with > 0.5% parasitaemia resulted in > 95% complete schizogony. Culture could be continued for 5-6 cycles without the addition of red cells. Criteria for staging the erythrocytic development of P. vivax in the first schizogonic cycle based on synchronous ex-vivo culture are presented. The asexual cycle was divided into 7 morphological stages tiny ring (0-6 h), small ring (6-12 h), large ring (12-18 h), early trophozoite (18-28 h), late trophozoite (28-36 h), early schizont (36-42 h) and mature schizont (42-48 h). This simple method of culturing P. vivax ex vivo is suitable for antimalarial susceptibility and immunoparasitology studies.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Parasitologia
/
Plasmodium vivax
/
Meios de Cultura
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Trans R Soc Trop Med Hyg
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Tailândia